What does chloroquine do in transfection

Discussion in 'Northwest Pharmacy' started by osty, 16-Mar-2020.

  1. valkiriy Moderator

    What does chloroquine do in transfection


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    Add 22 l of 25mM chloroquine to each 15cm dish for 10cm plate 7.8 l of chloroquinei.e. final concentration = 25 M. The cells should be transfected within 5-10’ of adding the chloroquine. 7. For 15cm plates, add 1.69ml of the transfection buffer to the DNA-CaCl2 solution by “bubbling”. Carefully transfer the transfection mix to the Lenti-X 293T packaging cells. Add the transfection mix dropwise being careful not to dislodge the cells. Incubate the cells for 18 h, or until the following morning. The following morning, carefully aspirate the media. Replace the media with 15 mL of DMEM complete. Chloroquine phosphate may cause an upset stomach. Take chloroquine phosphate with food. Follow the directions on your prescription label carefully, and ask your doctor or pharmacist to explain any part you do not understand. Use chloroquine phosphate exactly as directed. Do not use more or less of it or use it more often than prescribed by your.

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    What does chloroquine do in transfection

    Chloroquine Uses, Side Effects, Dosage & Interactions, Addgene General Transfection

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  7. Transfection is the process of introducing nucleic acids into eukaryotic cells by nonviral methods. Using various chemical or physical methods, this gene transfer technology enables the study of gene function and protein expression in a cellular environment.

    • Transfection Guide Overview of Transfection Methods Promega.
    • Chloroquine MedlinePlus Drug Information.
    • Glycerol shock or chloroquine? - Transfection and Transduction.

    How do you do transfection for lentivirus production. I use chloroquine, which inhibits DNA degradation by lysosomes by raising their pH - but this is toxic to the cell, so media must be. Chloroquine is an inhibitor of the lysosomal degradation of the DNA which is taken up by the cells, so as leelee said, transfection should have been successful, albeit at a slightly lower level than if you would have added the chloroquine. In my normal transfections, I never add chloroquine and get high efficiencies nevertheless. It was originally used as an antimalarial compound. It kills the erythrocytic forms of malaria parasites at all stages of development, but does not affect the sporozo ites. Keywords chloroquine diphosphate salt, c6628, c18h26cin3, dna transfection, tranfection efficiency, antigen-antibody complexes dissociate, denaturing red blood cell antigens,

     
  8. NEETRO Moderator

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  9. Hydroxychloroquine Oral Uses, Side Effects, Interactions, Pictures. Find patient medical information for Hydroxychloroquine Oral on WebMD including its uses, side effects and safety, interactions, pictures, warnings and user ratings.

    What are the side effects of hydroxychloroquine and.